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primescript reverse transcriptase mix  (TaKaRa)


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    Structured Review

    TaKaRa primescript reverse transcriptase mix
    Primescript Reverse Transcriptase Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 4921 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescript+reverse+transcriptase+mix/PrimeScript+Reverse+Transcriptase/pmc13041383-63-11-15
    Average 97 stars, based on 4921 article reviews
    primescript reverse transcriptase mix - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Synthesized:

    Article Title: Identification of a novel gene in Pseudomonas aeruginosa promotes persister formation by repressing translation and cell division
    Article Snippet: Total RNA was isolated from the collected bacterial cells with an RNeasy Mini kit (Tiangen Biotech, Beijing, China). .. The cDNA was synthesized from the total RNA by using the PrimeScript Reverse Transcriptase Mix (TaKaRa, China). ..

    Article Title: Identification of a novel gene in Pseudomonas aeruginosa promotes persister formation by repressing translation and cell division.
    Article Snippet: Total RNA was isolated from the collected bacterial cells with an RNeasy Mini kit (Tiangen Biotech, Beijing, China). .. The cDNA was synthesized from the total RNA by using the PrimeScript Reverse Transcriptase Mix (TaKaRa, China). ..

    Reverse Transcription:

    Article Title: Identification of a novel gene in Pseudomonas aeruginosa promotes persister formation by repressing translation and cell division
    Article Snippet: Total RNA was isolated from the collected bacterial cells with an RNeasy Mini kit (Tiangen Biotech, Beijing, China). .. The cDNA was synthesized from the total RNA by using the PrimeScript Reverse Transcriptase Mix (TaKaRa, China). ..

    Article Title: High-throughput sequencing identifies distinct fecal and mucosal gut microbiota correlating with different mucosal proteins
    Article Snippet: The 142 mRNA concentrations and purity were measured with an Eppendorf BioSpectrometer. .. After 143 reverse transcription with PrimeScript Reverse Transcriptase Mix (TaKaRa), which converted 144 the total RNA to cDNA, the expression of the BNNF, ZO1, TLR2, TLR4, AQP3, and AQP8genes 145 was determined with qPCR and SYBR Green technology on a Bio-Rad CFX96TM Q-PCR 146 instrument(Bio-Rad, USA) for each sample in duplicate. ..

    Article Title: Faecal and mucosal microbiota in patients with functional gastrointestinal disorders: Correlation with toll-like receptor 2/toll-like receptor 4 expression
    Article Snippet: Total mucosal RNA was extracted from the colonic biopsies using the TaKaRa MiniBEST Universal RNA Extraction Kit (TaKaRa, Japan), according to the manufacturer’s instructions. .. After reverse transcription with PrimeScript Reverse Transcriptase Mix (TaKaRa), the expression of TLR2 and TLR4 genes was determined by quantitative real-time polymerase chain reaction (qPCR) and SYBR Green technology on a Bio-Rad CFX96 Q-PCR instrument (Bio-Rad, United States) in duplicate. ..

    Article Title: Sox6 impairs the adipogenic commitment of mesenchymal stem cells by targeting lysyl oxidase and preadipocyte factor 1.
    Article Snippet: The commitment of mesenchymal stem cells (MSCs) to preadipocytes and the termination of differentiation to adipocytes are critical for maintaining systemic energy homeostasis.. However, our knowledge of the molecular mechanisms governing the commitment of MSCs to preadipocytes and the subsequent termination of their differentiation into adipocytes remain limited.. Additionally, the role of Sox6 sex-determining region Y (SRY)-box6 (Sox6), a transcription factor that regulates gene transcription, is reportedly involved in various cellular processes, including adipogenesis; however, its function in regulating preadipocyte development and the factors involved in the termination of adipogenic differentiation remain unexplored.

    Article Title: Identification of a novel gene in Pseudomonas aeruginosa promotes persister formation by repressing translation and cell division.
    Article Snippet: Total RNA was isolated from the collected bacterial cells with an RNeasy Mini kit (Tiangen Biotech, Beijing, China). .. The cDNA was synthesized from the total RNA by using the PrimeScript Reverse Transcriptase Mix (TaKaRa, China). ..

    Expressing:

    Article Title: High-throughput sequencing identifies distinct fecal and mucosal gut microbiota correlating with different mucosal proteins
    Article Snippet: The 142 mRNA concentrations and purity were measured with an Eppendorf BioSpectrometer. .. After 143 reverse transcription with PrimeScript Reverse Transcriptase Mix (TaKaRa), which converted 144 the total RNA to cDNA, the expression of the BNNF, ZO1, TLR2, TLR4, AQP3, and AQP8genes 145 was determined with qPCR and SYBR Green technology on a Bio-Rad CFX96TM Q-PCR 146 instrument(Bio-Rad, USA) for each sample in duplicate. ..

    Article Title: Faecal and mucosal microbiota in patients with functional gastrointestinal disorders: Correlation with toll-like receptor 2/toll-like receptor 4 expression
    Article Snippet: Total mucosal RNA was extracted from the colonic biopsies using the TaKaRa MiniBEST Universal RNA Extraction Kit (TaKaRa, Japan), according to the manufacturer’s instructions. .. After reverse transcription with PrimeScript Reverse Transcriptase Mix (TaKaRa), the expression of TLR2 and TLR4 genes was determined by quantitative real-time polymerase chain reaction (qPCR) and SYBR Green technology on a Bio-Rad CFX96 Q-PCR instrument (Bio-Rad, United States) in duplicate. ..

    Real-time Polymerase Chain Reaction:

    Article Title: High-throughput sequencing identifies distinct fecal and mucosal gut microbiota correlating with different mucosal proteins
    Article Snippet: The 142 mRNA concentrations and purity were measured with an Eppendorf BioSpectrometer. .. After 143 reverse transcription with PrimeScript Reverse Transcriptase Mix (TaKaRa), which converted 144 the total RNA to cDNA, the expression of the BNNF, ZO1, TLR2, TLR4, AQP3, and AQP8genes 145 was determined with qPCR and SYBR Green technology on a Bio-Rad CFX96TM Q-PCR 146 instrument(Bio-Rad, USA) for each sample in duplicate. ..

    Article Title: Faecal and mucosal microbiota in patients with functional gastrointestinal disorders: Correlation with toll-like receptor 2/toll-like receptor 4 expression
    Article Snippet: Total mucosal RNA was extracted from the colonic biopsies using the TaKaRa MiniBEST Universal RNA Extraction Kit (TaKaRa, Japan), according to the manufacturer’s instructions. .. After reverse transcription with PrimeScript Reverse Transcriptase Mix (TaKaRa), the expression of TLR2 and TLR4 genes was determined by quantitative real-time polymerase chain reaction (qPCR) and SYBR Green technology on a Bio-Rad CFX96 Q-PCR instrument (Bio-Rad, United States) in duplicate. ..

    SYBR Green Assay:

    Article Title: High-throughput sequencing identifies distinct fecal and mucosal gut microbiota correlating with different mucosal proteins
    Article Snippet: The 142 mRNA concentrations and purity were measured with an Eppendorf BioSpectrometer. .. After 143 reverse transcription with PrimeScript Reverse Transcriptase Mix (TaKaRa), which converted 144 the total RNA to cDNA, the expression of the BNNF, ZO1, TLR2, TLR4, AQP3, and AQP8genes 145 was determined with qPCR and SYBR Green technology on a Bio-Rad CFX96TM Q-PCR 146 instrument(Bio-Rad, USA) for each sample in duplicate. ..

    Article Title: Faecal and mucosal microbiota in patients with functional gastrointestinal disorders: Correlation with toll-like receptor 2/toll-like receptor 4 expression
    Article Snippet: Total mucosal RNA was extracted from the colonic biopsies using the TaKaRa MiniBEST Universal RNA Extraction Kit (TaKaRa, Japan), according to the manufacturer’s instructions. .. After reverse transcription with PrimeScript Reverse Transcriptase Mix (TaKaRa), the expression of TLR2 and TLR4 genes was determined by quantitative real-time polymerase chain reaction (qPCR) and SYBR Green technology on a Bio-Rad CFX96 Q-PCR instrument (Bio-Rad, United States) in duplicate. ..



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